The DIO2 Polyclonal Antibody (PACO19573) is a key tool for researchers studying DIO2, an enzyme involved in the conversion of thyroid hormone. This antibody, produced in rabbits, is highly specific to human DIO2 protein and has been validated for use in Western blotting applications. By targeting the DIO2 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for investigations in endocrinology and metabolism research.
DIO2 is a critical player in the regulation of thyroid hormone levels, impacting metabolism, growth, and development. Dysregulation of DIO2 function has been linked to thyroid disorders, obesity, and other metabolic conditions. By studying the role of DIO2 using this antibody, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets for treatment.
Antibody Name:
DIO2 Antibody (PACO19573)
Antibody SKU:
PACO19573
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:25-1:100
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide of human DIO2
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using PACO19573(DIO2 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
The protein encoded by this gene belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It is highly expressed in the thyroid, and may contribute significantly to the relative increase in thyroidal T3 production in patients with Graves disease and thyroid adenomas. This protein contains selenocysteine (Sec) residues encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in multiple transcript variants encoding different isoforms.
Synonyms:
deiodinase, iodothyronine, type II
UniProt Protein Function:
DIO2: Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development. Belongs to the iodothyronine deiodinase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:EC 1.97.1.10; Oxidoreductase; Membrane protein, integral
Chromosomal Location of Human Ortholog: 14q24.2-q24.3
Cellular Component: membrane; plasma membrane
Molecular Function:selenium binding; thyroxine 5'-deiodinase activity; ubiquitin protein ligase binding
Biological Process: thyroid hormone generation; thyroid hormone metabolic process
NCBI Summary:
The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the conversion of prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4) to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by outer ring 5'-deiodination. This gene is widely expressed, including in thyroid, placenta, pituitary and brain. It is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues. It has also been reported to be highly expressed in thyroids of patients with Graves disease, and in follicular adenomas. The intrathyroidal T4 to T3 conversion by this enzyme may contribute significantly to the relative increase in thyroidal T3 production in these patients. This protein is a selenoprotein containing the rare selenocysteine (Sec) amino acid at its active site, and may contain additional Sec residues. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2016]
