Description
DIO3 Antibody (CAB6900)
The DIO3 Antibody (CAB6900) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been thoroughly validated for use in Western blot applications. By binding specifically to the Dio3 protein, researchers can accurately detect and analyze its expression in various cell types, making it an essential component in studies related to endocrinology and metabolism.Dio3, also referred to as type 3 deiodinase, is an enzyme that regulates the levels of active thyroid hormones in the body, impacting metabolic processes and overall physiological functions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | DIO3 Antibody |
| SKU: | CAB6900 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | HFLG RRRR GQPE PEVE LNSE GEEV PPDD PPIC VSDD NRLC TLAS LKAV WHGQ KLDF FKQA HEGG PAPN SEVV LPDG FQSQ HILD YAQG NRPL VLNF GSCT U | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Synonyms: | D3, 5DIII, TXDI3, DIOIII, DIO3 |
| Positive Sample: | SW620, HepG2, MCF7 |
| Cellular Localization: | Cell Membrane, Endosome Membrane, Single-Pass Type Ii Membrane Protein. |
| Calculated MW: | 34kDa |
| Observed MW: | 34kDa |
The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in pregnant uterus, placenta, fetal and neonatal tissues, and thought to prevent premature exposure of developing fetal tissues to adult levels of thyroid hormones. It regulates circulating fetal thyroid hormone concentrations, and thus plays a critical role in mammalian development. Knockout mice lacking this gene exhibit abnormalities related to development and reproduction, and increased activity of this enzyme in infants with hemangiomas causes severe hypothyroidism. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.
| Purification Method | Affinity purification |
| Gene ID | 1735 |
| RRID | AB_2767459 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of various lysates using DIO3 Rabbit pAb (CAB6900) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s. |
