The DIRAS3 Antibody (CAB7950) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.Diras3, also known as Dual Ras Homolog 3, is involved in signaling pathways that regulate cell proliferation, survival, and differentiation. By targeting the Diras3 protein, researchers can gain insights into its function and impact on different cellular functions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
DIRAS3 Antibody
SKU:
CAB7950
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ARHI, NOEY2, DIRAS3
Positive Sample:
NIH/3T3, Mouse liver, Mouse kidney, Rat liver
Cellular Localization:
Cell Membrane, Cytoplasmic Side, Lipid-Anchor.
Calculated MW:
26kDa
Observed MW:
30kDa
This gene encodes a member of the ras superfamily. This gene is imprinted gene with monoallelic expression of the paternal allele which is associated with growth suppression. The encoded protein acts as a tumor suppressor whose function is abrogated in many ovarian and breast cancers. This protein may also play a role autophagy in certain cancer cells by regulating the autophagosome initiation complex.
Purification Method
Affinity purification
Gene ID
9077
RRID
AB_2769173
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DIRAS3 Rabbit pAb (CAB7950) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
