The DLGAP5 Antibody (CAB2197) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity for DLGAP5 in human samples and is validated for use in Western blotting applications. By binding to the DLGAP5 protein, this antibody allows for accurate detection and analysis in various cell types, making it ideal for studies in cell biology and oncology research.DLGAP5, also known as hGTSE-1, plays a critical role in regulating cell cycle progression and mitotic spindle assembly, making it a key player in cellular division and proliferation.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
DLGAP5 Antibody
SKU:
CAB2197
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DLG7, HURP, DLGAP5
Positive Sample:
K-562
Cellular Localization:
Cytoplasm, Nucleus, Cytoskeleton, Spindle.
Calculated MW:
95kDa
Observed MW:
120kDa
Predicted to enable microtubule binding activity. Predicted to be involved in several processes, including centrosome localization; kinetochore assembly; and mitotic spindle organization. Located in several cellular components, including centrosome; cytosol; and mitochondrion.
Purification Method
Affinity purification
Gene ID
9787
RRID
AB_2764214
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from K562 cells, using DLGAP5 Rabbit pAb (CAB2197) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using DLGAP5 Rabbit pAb (CAB2197). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
