The DLL4 Polyclonal Antibody (CAB24862) is a high-quality antibody developed for reliable detection and analysis of target proteins. Produced in rabbits, this antibody is highly specific to human DLL4 and has been validated for use in Western blot applications. By binding to DLL4, this antibody allows for the detection and analysis of DLL4 expression in various cell types, making it an essential asset for studies in cancer biology, developmental biology, and vascular biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
DLL4 Polyclonal Antibody
SKU:
CAB24862
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DLL4, AOS6, hdelta2, delta-like protein 4
Positive Sample:
293T transfected with DLL4
Cellular Localization:
Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
74kDa
Observed MW:
90kDa
This gene is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.
Purification Method
Affinity purification
Gene ID
54567
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with DLL4 using DLL4 Rabbit pAb (CAB24862) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.