The DOCK7 Antibody (CAB17236) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, raised in rabbits, is highly specific for human samples and is validated for use in various applications, including Western blot and immunofluorescence.DOCK7 is known to regulate the activity of small GTPases, such as Rac1 and Cdc42, which are key players in cell migration and adhesion. Dysregulation of DOCK7 has been implicated in various diseases, including cancer and neurological disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DOCK7 Antibody
SKU:
CAB17236
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ZIR2, DEE23, EIEE23, DOCK7
Positive Sample:
HeLa, Jurkat, U-87MG, Mouse brain, Mouse ovary, Rat brain
Cellular Localization:
Axon, Growth Cone, Neuron Projection.
Calculated MW:
243kDa
Observed MW:
250kDa
The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that plays a role in axon formation and neuronal polarization. The encoded protein displays GEF activity toward RAC1 and RAC3 Rho small GTPases but not toward CDC42. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
85440
RRID
AB_2769209
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DOCK7 Rabbit pAb (CAB17236) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
