The DOK4 Antibody (CAB0226) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to Dok-4, this antibody enables accurate detection and analysis of the protein in a variety of cell types, making it an essential asset for studies in molecular biology, cell signaling, and cancer research.Dok-4 plays a crucial role in mediating signal transduction events within cells, particularly in response to growth factors and cytokines.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DOK4 Antibody
SKU:
CAB0226
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IRS5, IRS-5, DOK4
Positive Sample:
Mouse heart, Mouse kidney, Rat heart, Rat kidney
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
37kDa
Observed MW:
37kDa
Predicted to be involved in positive regulation of MAPK cascade and transmembrane receptor protein tyrosine kinase signaling pathway. Predicted to act upstream of or within nervous system development. Predicted to be located in cytosol. Predicted to be active in cytoplasm.
Purification Method
Affinity purification
Gene ID
55715
RRID
AB_2757039
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DOK4 Rabbit pAb (CAB0226) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using DOK4 Rabbit pAb (CAB0226) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of PC-12 cells using DOK4 Rabbit pAb (CAB0226) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
