Description
DOK3 Antibody (PACO49526)
The DOK3 Polyclonal Antibody (PACO49526) is a key tool for researchers studying DOK3, a protein involved in immune signaling pathways. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in a variety of applications, including Western blotting. By binding to DOK3, this antibody enables precise detection and analysis of the protein in different cell types, making it an essential tool for studies in immunology, cancer research, and beyond.
DOK3, also known as downstream of tyrosine kinase 3, plays a critical role in regulating immune responses and cell growth. Its involvement in signaling pathways associated with cancer and autoimmune diseases makes it a promising target for therapeutic interventions. By investigating the functions of DOK3, researchers can gain valuable insights into the development of novel treatments for these diseases.
| Antibody Name: | DOK3 Antibody (PACO49526) |
| Antibody SKU: | PACO49526 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Recombinant Human Docking protein 3 protein (1-146AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Mouse heart tissue, Human placenta tissue. All lanes: DOK3 antibody at 3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 54, 24, 36, 25 kDa. Observed band size: 54 kDa.. |
 | Immunofluorescent analysis of A549 cells using PACO49526 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Immunohistochemistry of paraffin-embedded human tonsil tissue using PACO49526 at dilution of 1:100. |
| Background: | DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL1 function (By similarity). |
| Synonyms: | Docking protein 3 (Downstream of tyrosine kinase 3), DOK3 |
| UniProt Protein Function: | DOK3: docking proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with SHIP. May modulate Abl function. Upon tyrosine phosphorylation, Dok3 interacts with CSK and SHIP via their SH2 domains. Both pY381 and pY398 are required for interaction with SHIP. Only pY381 is required for interaction with CSK. Binds ABL through the PTB domain and in a kinase-dependent manner. On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Does not interact with RasGAP. Four isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 5q35.3 Molecular Function:protein binding |
| UniProt Code: | Q7L591 |
| NCBI GenInfo Identifier: | 84029599 |
| NCBI Gene ID: | 79930 |
| NCBI Accession: | Q7L591.2 |
| UniProt Secondary Accession: | Q7L591,Q8N864, Q9BQB3, Q9H666, E9PAT0, H7BXS0, |
| UniProt Related Accession: | Q7L591 |
| Molecular Weight: | 24,737 Da |
| NCBI Full Name: | Docking protein 3 |
| NCBI Synonym Full Names: | docking protein 3 |
| NCBI Official Symbol: | DOK3Â Â |
| NCBI Official Synonym Symbols: | DOKLÂ Â |
| NCBI Protein Information: | docking protein 3 |
| UniProt Protein Name: | Docking protein 3 |
| UniProt Synonym Protein Names: | Downstream of tyrosine kinase 3 |
| Protein Family: | Docking protein |
| UniProt Gene Name: | DOK3Â Â |
| UniProt Entry Name: | DOK3_HUMAN |
