The DOK5 Antibody (CAB13726) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards DOK5 in human samples, making it an ideal choice for Western blot applications.DOK5, also known as downstream of tyrosine kinase 5, is a key player in intracellular signaling pathways that regulate cell proliferation and differentiation. Dysregulation of DOK5 has been linked to various diseases, including cancer and metabolic disorders, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
DOK5 Antibody
SKU:
CAB13726
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IRS6, IRS-6, C20orf180, DOK5
Positive Sample:
Mouse kidney, Mouse liver, Rat skeletal muscle
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
35kDa
Observed MW:
30kDa
The protein encoded by this gene is a member of the DOK family of membrane proteins, which are adapter proteins involved in signal transduction. The encoded protein interacts with phosphorylated receptor tyrosine kinases to mediate neurite outgrowth and activation of the MAP kinase pathway. Unlike other DOK family proteins, this protein does not interact with RASGAP. This protein is up-regulated in patients with systemic sclerosis and is associated with fibrosis induced by insulin-like growth factor binding protein 5. Alternative splicing of this gene results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
55816
RRID
AB_2760585
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using DOK5 Rabbit pAb (CAB13726) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
