The DPYS Antibody (CAB6368) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the dihydropyrimidinase protein, this antibody allows for the detection and analysis of dihydropyrimidinase levels in various cell types, making it ideal for studies in biochemistry and molecular biology.Dihydropyrimidinase plays a crucial role in the breakdown of pyrimidine nucleotides, which are essential for DNA and RNA synthesis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
DPYS Antibody
SKU:
CAB6368
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DHP, DHPase, DPYS
Positive Sample:
Mouse liver, Mouse kidney
Cellular Localization:
Cytosol, Extracellular Exosome.
Calculated MW:
57kDa
Observed MW:
54kDa
Dihydropyrimidinase catalyzes the conversion of 5,6-dihydrouracil to 3-ureidopropionate in pyrimidine metabolism. Dihydropyrimidinase is expressed at a high level in liver and kidney as a major 2.5-kb transcript and a minor 3.8-kb transcript. Defects in the DPYS gene are linked to dihydropyrimidinuria.
Purification Method
Affinity purification
Gene ID
1807
RRID
AB_2766970
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DPYS Rabbit pAb (CAB6368) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
