The DTX3 Polyclonal Antibody (PAC063179) is a valuable tool for researchers studying DTX3, a protein involved in various cellular processes such as apoptosis, cell cycle regulation, and DNA repair. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the DTX3 protein, this antibody enables accurate detection and analysis in a range of cell types, making it ideal for studies in cancer biology, molecular biology, and cell signaling pathways.
DTX3, also known as deltex E3 ubiquitin ligase 3, plays a crucial role in controlling protein stability and turnover, making it a key player in cellular homeostasis and disease development. Its involvement in regulating cell growth, differentiation, and survival makes it a potential target for therapeutic interventions in cancer and other diseases. Understanding the function of DTX3 is essential for developing targeted therapies that exploit its roles in cellular processes and signaling pathways.
IHC image of PACO63179 diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of SH-SY5Y cells with PACO63179 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context (By similarity). Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.
DTX3: Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity. Belongs to the Deltex family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.-Chromosomal Location of Human Ortholog: 12q13.3Molecular Function: protein binding
UniProt Protein Details:
NCBI Summary:
DTX3 functions as an E3 ubiquitin ligase (Takeyama et al., 2003 [PubMed 12670957]).[supplied by OMIM, Nov 2009]
