The DUT Monoclonal Antibody (CAB23563) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, developed using advanced technologies, is highly specific and sensitive for detecting dUTPase in various biological samples.DUT, or deoxyuridine triphosphatase, is essential for maintaining genomic stability by preventing the incorporation of uracil into DNA. Dysregulation of dUTPase activity has been linked to genomic instability and diseases such as cancer. Therefore, understanding the function and regulation of dUTPase is vital for advancing research in oncology and DNA repair mechanisms.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
DUT Monoclonal Antibody
SKU:
CAB23563
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC3127
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
BMFDMS, dUTPase, DUT
Positive Sample:
Jurkat
Cellular Localization:
Mitochondrion, Nucleus.
Calculated MW:
27kDa
Observed MW:
23kDa
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
Purification Method
Affinity purification
Gene ID
1854
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Jurkat cells, using DUT Rabbit mAb (CAB23563) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
