The DYNLL1 Monoclonal Antibody (CAB4353) is a high-quality antibody developed for reliable detection and analysis of target proteins. Cytoplasmic dyneins are large enzyme complexes with a molecular mass of about 1,200 kD. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain, which contains a varying number of accessory intermediate chains. The complex is involved in intracellular transport and motility. The protein described in this record is a light chain and exists as part of this complex but also physically interacts with and inhibits the activity of neuronal nitric oxide synthase. Binding of this protein destabilizes the neuronal nitric oxide synthase dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. Alternate transcriptional splice variants have been characterized. RRID AB_2863246 Gene ID 8655 Swiss Prot Synonym LC8; PIN; DLC1; DLC8; LC8a; DNCL1; hdlc1; DNCLC1; DYNLL1
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
DYNLL1 Monoclonal Antibody
SKU:
CAB4353
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0975
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
HeLa, U-87MG, Mouse testis, Mouse brain, Rat lung, Rat testis
Cellular Localization:
Cytoplasm, Mitochondrion, Nucleus, Cytoskeleton.
Calculated MW:
10kDa
Observed MW:
10kDa
Cytoplasmic dyneins are large enzyme complexes with a molecular mass of about 1,200 kD. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain, which contains a varying number of accessory intermediate chains. The complex is involved in intracellular transport and motility. The protein described in this record is a light chain and exists as part of this complex but also physically interacts with and inhibits the activity of neuronal nitric oxide synthase. Binding of this protein destabilizes the neuronal nitric oxide synthase dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. Alternate transcriptional splice variants have been characterized. RRID AB_2863246 Gene ID 8655 Swiss Prot Synonym LC8; PIN; DLC1; DLC8; LC8a; DNCL1; hdlc1; DNCLC1; DYNLL1
Purification Method:
Affinity purification
Gene ID:
8655
RRID:
AB_2863246
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using DYNLL1 Rabbit mAb (CAB4353) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human colon using DYNLL1 Rabbit mAb (CAB4353) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
