E-Tag Monoclonal Antibody is a premium monoclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, WB, IP, this antibody ensures consistent, reproducible results across multiple experimental platforms. Conveniently packaged in 50ul format to meet your experimental needs. For optimal performance, store at Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
Product Name:
E-Tag Monoclonal Antibody (MACO0659)
SKU:
MACO0659
Size:
50μl
Isotype:
IgG1
Host Species:
Mouse
Reactivity:
All
Immunogen:
GAPVPYPDPLEPRC synthetic peptide conjugate to KLH
Form:
Liquid
Tested Applications:
ELISAWBIP
Recommended Dilution:
ELISA 1:4000-1:8000, WB 1:1000-1:320000, IP 1μg-5μg
Western Blot Positive WB detected in: E-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng All lanes: E-Tag antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa
Western Blot Positive WB detected in: E-tagged fusion protein All lanes: E-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa
Immunoprecipitating E-Tag in 293F transfected whole cell lysate Lane 1: Mouse control IgG (1µg) instead of MACO0659 in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: MACO0659 (5µg) + 293F transfected whole cell lysate (500µg) Lane 3: 293F transfected whole cell lysate (20µg)
ELISA E-Tag antibody in E-tagged fusion protein and Control protein (no E-tag) Antigen at dilution of 1µg/ml E-Tag antibody at (0-0.15µg) Goat polyclonal to Mouse IgG at 1/50000 dilution
Western Blot Positive WB detected in: 1-3 lanes: E‐tagged fusion protein, 4-5 lanes: Recombinant protein without E-tagged, 6 lane: BSA-E-Tag All lanes: E-Tag antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 30, 68, 55, 67-157kDa Observed band size: 30, 68, 55, 67-157kDa
