The EDC4 Antibody (CAB15418) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in various applications including Western blot, immunofluorescence, and immunoprecipitation.EDC4, also known as enhancer of mRNA decapping, is a crucial factor in mRNA degradation and translation repression processes, making it a vital player in cellular homeostasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
EDC4 Antibody
SKU:
CAB15418
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GE1, Ge-1, RCD8, HEDL5, HEDLS, RCD-8, EDC4
Positive Sample:
Jurkat, Mouse liver
Cellular Localization:
Cytoplasm, Nucleus, P-Body.
Calculated MW:
152kDa
Observed MW:
160-180kDa
Predicted to be involved in deadenylation-independent decapping of nuclear-transcribed mRNA. Located in P-body and nucleoplasm.
Purification Method
Affinity purification
Gene ID
23644
RRID
AB_2762326
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using EDC4 Rabbit pAb (CAB15418) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
