The EEFSEC Antibody (CAB17749) is a high-quality antibody developed for reliable detection and analysis of target proteins. Raised in rabbits, this antibody is highly specific and reacts with human samples, making it ideal for various research applications. Validated for use in Western blotting, this antibody binds to EEFSEC, allowing for accurate detection and analysis in a variety of cell types.EEFSEC plays a crucial role in selenium metabolism, which is essential for proper cellular function and antioxidant defense. Dysregulation of EEFSEC has been linked to various health conditions, including cancer, cardiovascular disease, and neurodegenerative disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
EEFSEC Antibody
SKU:
CAB17749
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SELB, EFSEC, EEFSEC
Positive Sample:
Raji, HepG2, HeLa
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
65kDa
Observed MW:
72kDa
Predicted to enable translation elongation factor activity. Predicted to be involved in selenocysteine incorporation. Predicted to be located in cytoplasm and nucleus. Predicted to be part of ribonucleoprotein complex.
Purification Method
Affinity purification
Gene ID
60678
RRID
AB_2769268
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using EEFSEC Rabbit pAb (CAB17749) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.