The Egr4 Antibody (CAB16369) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the EGR4 protein, researchers can accurately detect and analyze its expression in various cell types.EGR4 is known to play a role in cell growth, differentiation, and response to stress, making it a key target for studies in cancer research, developmental biology, and neurobiology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Egr4 Antibody
SKU:
CAB16369
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NGFIC, NGF1-C, NGFI-C, pAT133, Egr4
Positive Sample:
Mouse brain, Mouse kidney
Cellular Localization:
Nucleus.
Calculated MW:
62kDa
Observed MW:
58kDa
Predicted to enable DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in positive regulation of transcription by RNA polymerase II. Predicted to be located in nucleus. Is expressed in several structures, including central nervous system; genitourinary system; and tooth. Orthologous to human EGR4 (early growth response 4).
Purification Method
Affinity purification
Gene ID
13656
RRID
AB_2769280
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Egr4 Rabbit pAb (CAB16369) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
