The EIF2AK1 Antibody (CAB14119) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. RRID AB_2760975 Gene ID 27102 Swiss Prot Synonym HCR; HRI; hHRI; LEMSPAD; EIF2AK1
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
EIF2AK1 Antibody
SKU:
CAB14119
Size:
100μL, 20μL
Reactivity:
Human, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HCR, HRI, hHRI, LEMSPAD, EIF2AK1
Positive Sample:
K-562, Rat liver
Cellular Localization:
Cytoplasm.
Calculated MW:
71kDa
Observed MW:
71kDa
The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. RRID AB_2760975 Gene ID 27102 Swiss Prot Synonym HCR; HRI; hHRI; LEMSPAD; EIF2AK1
Purification Method:
Affinity purification
Gene ID:
27102
RRID:
AB_2760975
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from K-562 cells, using EIF2AK1 Rabbit pAb (CAB14119) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from Rat liver, using EIF2AK1 Rabbit pAb (CAB14119) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 30s.
