The EIF2AK1 Antibody (CAB3415) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications. By binding specifically to the EIF2AK1 protein, this antibody enables accurate detection and analysis in various cell types, making it an essential asset for studies in molecular biology and cancer research.EIF2AK1, also known as PKR-like endoplasmic reticulum kinase (PERK), plays a pivotal role in the integrated stress response pathway, which is essential for maintaining cellular homeostasis under stressful conditions such as nutrient deprivation or accumulation of misfolded proteins.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
EIF2AK1 Antibody
SKU:
CAB3415
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HCR, HRI, hHRI, LEMSPAD, K1
Positive Sample:
293T, HeLa, SH-SY5Y
Cellular Localization:
Cytoplasm.
Calculated MW:
71kDa
Observed MW:
74kDa
The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
27102
RRID
AB_2765100
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using [KO Validated] EIF2AK1 Rabbit pAb (CAB3415) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of lysates from wild type(WT) and EIF2AK1 Rabbit pAb knockout (KO) 293T cells, using [KO Validated] EIF2AK1 Rabbit pAb (CAB3415) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
