The EIF2B4 Antibody (CAB18203) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for Western blot applications. By binding to the EIF2B4 protein, this antibody enables the detection and analysis of EIF2B4 in various cell types, facilitating investigations in molecular biology and cell signaling pathways.EIF2B4, a subunit of the eukaryotic translation initiation factor 2B (eIF2B) complex, plays a crucial role in the control of protein synthesis in response to cellular stress signals. Dysregulation of EIF2B4 has been associated with a variety of human diseases, including neurodegenerative disorders and certain types of cancer.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
EIF2B4 Antibody
SKU:
CAB18203
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
8890
RRID
AB_2861980
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using EIF2B4 Rabbit pAb (CAB18203) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of L929 cells using EIF2B4 Rabbit pAb (CAB18203) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
