The eIF2B epsilon (EIF2B5) Antibody (CAB10263) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications. By binding to the EIF2B5 protein, this antibody allows for the detection and analysis of EIF2B5 expression in a variety of cell types, making it ideal for studies in molecular biology and protein synthesis research.EIF2B5 is a critical component of the translation initiation machinery, playing a key role in protein synthesis regulation.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
eIF2B epsilon (EIF2B5) Antibody
SKU:
CAB10263
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter.
Purification Method
Affinity purification
Gene ID
8893
RRID
AB_2757791
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using eIF2B epsilon (EIF2B5) Rabbit pAb (CAB10263) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of L929 cells using eIF2B epsilon (eIF2B epsilon (EIF2B5)) Rabbit pAb (CAB10263) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
