The eIF3B Monoclonal Antibody (CAB9143) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced from high-quality rabbit monoclonal cells, exhibits strong binding specificity to human EIF3B protein, making it ideal for Western blot applications. EIF3B is essential for protein synthesis and is involved in regulating cell growth, proliferation, and differentiation. Dysregulation of EIF3B has been linked to various diseases, including cancer, making it a promising target for therapeutic interventions. By using the EIF3B Rabbit Monoclonal Antibody, researchers can gain valuable insights into the function and expression of EIF3B in different cell types, advancing our understanding of its role in cellular processes and disease pathology.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
eIF3B Monoclonal Antibody
SKU:
CAB9143
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1448
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Enables RNA binding activity. Contributes to translation initiation factor activity. Involved in several processes, including IRES-dependent viral translational initiation; translational initiation; and viral translational termination-reinitiation. Located in extracellular exosome. Part of eukaryotic translation initiation factor 3 complex.
Purification Method
Affinity purification
Gene ID
8662
RRID
AB_2863668
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using eIF3B Rabbit mAb (CAB9143) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Confocal imaging of NIH/3T3 cells using eIF3B Rabbit mAb (CAB9143, dilution 1:100) followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (CABS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) (CABS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
