The ELAC2 Antibody (CAB7128) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed in rabbits, shows high specificity towards human samples and has been validated for use in Western blot applications. By targeting the ELAC2 protein, researchers can accurately detect and analyze its expression in various cell types, making it an ideal choice for studies in molecular biology and mitochondrial research.ELAC2, also known as Endoribonuclease ZC3H12A, plays a crucial role in maintaining RNA integrity, ensuring proper mitochondrial function, and regulating gene expression.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ELAC2 Antibody
SKU:
CAB7128
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ELC2, HPC2, COXPD17, ELAC2
Positive Sample:
MCF7, NCI-H460
Cellular Localization:
Mitochondrion, Nucleus.
Calculated MW:
92kDa
Observed MW:
105kDa
The protein encoded by this gene has a C-terminal domain with tRNA 3′ processing endoribonuclease activity, which catalyzes the removal of the 3' trailer from precursor tRNAs. The protein also interacts with activated Smad family member 2 (Smad2) and its nuclear partner forkhead box H1 (also known as FAST-1), and reduced expression can suppress transforming growth factor-beta induced growth arrest. Mutations in this gene result in an increased risk of prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
60528
RRID
AB_2767683
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ELAC2 Rabbit pAb (CAB7128) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Rat testis using ELAC2 Rabbit pAb (CAB7128) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using ELAC2 Rabbit pAb (CAB7128) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
