The ELP4 Antibody (PACO59337) is a crucial tool for researchers studying the ELP4 protein and its function in gene expression regulation. This polyclonal antibody, produced in rabbits, has been extensively validated for use in various applications, including immunofluorescence and immunohistochemistry.ELP4 is a component of the Elongator complex, which plays a vital role in transcription elongation and tRNA modification. Dysregulation of ELP4 has been linked to neurodevelopmental disorders and cancer, making it an important target for investigation in these fields.
The ELP4 Antibody specifically binds to ELP4, allowing for precise detection and localization within cells, making it an invaluable tool for researchers in molecular biology and disease pathology.By utilizing the ELP4 Antibody, researchers can gain valuable insights into the function of ELP4 and its role in various cellular processes, ultimately contributing to a better understanding of its implications in disease development and potential therapeutic interventions.
IHC image of PACO59337 diluted at 1:600 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of MCF-7 cells with PACO59337 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO59337 diluted at 1:600 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.
ELP4: Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. Belongs to the ELP4 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Unknown functionChromosomal Location of Human Ortholog: 11p13Cellular Component: nucleoplasm; transcription elongation factor complex; Elongator holoenzyme complex; cytoplasm; histone acetyltransferase complexMolecular Function: protein binding; histone acetyltransferase activity; phosphorylase kinase regulator activityBiological Process: regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; RNA elongation from RNA polymerase II promoter; regulation of protein kinase activityDisease: Aniridia
UniProt Protein Details:
NCBI Summary:
This gene encodes a component of the six subunit elongator complex, a histone acetyltransferase complex that associates directly with RNA polymerase II during transcriptional elongation. The human gene can partially complement sensitivity phenotypes of yeast ELP4 deletion mutants. This gene has also been associated with Rolandic epilepsy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
