The ELSPBP1 Antibody (CAB15882) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene belongs to the sperm-coating protein family of epididymal origin. This protein and its canine homolog are the first known examples of proteins with four tandemly arranged fibronectin type 2 (Fn2) domains in the Fn2-module protein family.
This antibody is validated for use in WB, ELISA, IF-P applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
ELSPBP1 Antibody
SKU:
CAB15882
Size:
100μL, 20μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISAIF-P
Recommended Dilution:
WB
1:500 - 1:2000
IF-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
E12, HE12, EL149, EDDM12, ELSPBP1
Positive Sample:
Mouse testis, Rat testis
Cellular Localization:
Secreted.
Calculated MW:
26kDa
Observed MW:
26kDa
The protein encoded by this gene belongs to the sperm-coating protein family of epididymal origin. This protein and its canine homolog are the first known examples of proteins with four tandemly arranged fibronectin type 2 (Fn2) domains in the Fn2-module protein family.
Purification Method
Affinity purification
Gene ID
64100
RRID
AB_2763312
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ELSPBP1 Rabbit pAb (CAB15882) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of paraffin-embedded rat testis using ELSPBP1 Rabbit pAb (CAB15882) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
