The EMCN Antibody (CAB8494) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human endomucin and is suitable for use in various applications, including immunohistochemistry and flow cytometry.Endomucin is known for its role in maintaining vascular integrity and regulating angiogenesis, making it a key target in studies of vascular biology and cardiovascular diseases. By targeting endomucin with this antibody, researchers can accurately detect and analyze the protein in different cell types and tissue samples, providing valuable insights into its function and potential therapeutic applications.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
EMCN Antibody
SKU:
CAB8494
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EMCN2, MUC14, Endomucin
Positive Sample:
MCF7, NIH/3T3
Cellular Localization:
Cell Membrane, Membrane, Secreted, Single-Pass Type I Membrane Protein, Single-Pass Type I Membrane Protein.
Calculated MW:
27kDa
Observed MW:
35kDa
EMCN is a mucin-like sialoglycoprotein that interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix (Kinoshita et al., 2001 [PubMed 11418125]).
Purification Method
Affinity purification
Gene ID
51705
RRID
AB_2769312
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Endomucin Rabbit pAb (CAB8494) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
