The ENPP3 Antibody (CAB14763) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the ENPP3 protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for investigations in the fields of immunology and cancer research.ENPP3 is a cell surface enzyme that plays a crucial role in regulating extracellular nucleotide and nucleoside levels, thereby influencing various physiological and pathological processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ENPP3 Antibody
SKU:
CAB14763
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
B10, NPP3, PDNP3, CD203c, PD-IBETA, ENPP3
Positive Sample:
DU145, HeLa, HT-29, Mouse lung, Rat liver
Cellular Localization:
Membrane, Secreted, Single-Pass Type Ii Membrane Protein.
Calculated MW:
100kDa
Observed MW:
110kDa
The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver. The human mRNA is expressed in glioma cells, prostate, and uterus. Expression of the human protein has been detected in uterus, basophils, and mast cells. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene.
Purification Method
Affinity purification
Gene ID
5169
RRID
AB_2761639
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ENPP3 Rabbit pAb (CAB14763) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
