The Epac1/RAPGEF3 Monoclonal Antibody (CAB4149) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables guanyl-nucleotide exchange factor activity and protein domain specific binding activity. Involved in several processes, including positive regulation of protein modification process
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Epac1/RAPGEF3 Monoclonal Antibody
SKU:
CAB4149
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0908
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:1000 - 1:2000
IHC-P
1:100 - 1:400
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements
Synonyms:
EPAC, Epac1/RAPGEF3
Positive Sample:
Mouse lung, Mouse brain, Mouse kidney, Rat brain
Cellular Localization:
Endomembrane System.
Calculated MW:
104kDa
Observed MW:
100kDa
Enables guanyl-nucleotide exchange factor activity and protein domain specific binding activity. Involved in several processes, including positive regulation of protein modification process
Purification Method
Affinity purification
Gene ID
10411
RRID
AB_2863197
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Epac1/RAPGEF3 Rabbit mAb (CAB4149) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Human placenta using Epac1/RAPGEF3 Rabbit mAb (CAB4149) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
