The Epac1/RAPGEF3 Monoclonal Antibody (CAB4149) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has been validated for use in techniques like Western blotting, immunohistochemistry, and immunofluorescence, allowing for accurate detection and analysis of ePAC1 expression in various cell types.ePAC1, also known as exchange protein directly activated by cAMP 1, plays a crucial role in regulating intracellular cAMP levels and downstream signaling cascades that impact processes like cell growth, differentiation, and metabolism.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Epac1/RAPGEF3 Monoclonal Antibody
SKU:
CAB4149
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0908
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements
Synonyms:
EPAC, Epac1/RAPGEF3
Positive Sample:
Mouse lung, Mouse brain, Mouse kidney, Rat brain
Cellular Localization:
Endomembrane System.
Calculated MW:
104kDa
Observed MW:
100kDa
Enables guanyl-nucleotide exchange factor activity and protein domain specific binding activity. Involved in several processes, including positive regulation of protein modification process
Purification Method
Affinity purification
Gene ID
10411
RRID
AB_2863197
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Epac1/RAPGEF3 Rabbit mAb (CAB4149) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Human placenta using Epac1/RAPGEF3 Rabbit mAb (CAB4149) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
