The EPHA3 Antibody (CAB8414) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity for human samples, making it suitable for use in Western blotting and immunohistochemistry applications. By targeting the EPHA3 protein, this antibody allows for the detection and analysis of EPHA3 expression in various cell types, providing insights into its role in cellular processes.EPHA3 has been implicated in a wide range of biological functions, including cell adhesion, migration, and survival, making it a promising target for investigations in developmental biology, neurobiology, and cancer research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
EPHA3 Antibody
SKU:
CAB8414
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EK4, ETK, HEK, ETK1, HEK4, TYRO4, EPHA3
Positive Sample:
SW620, Mouse lung, Rat heart
Cellular Localization:
Cell Membrane, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
110kDa
Observed MW:
110kDa
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene.
Purification Method
Affinity purification
Gene ID
2042
RRID
AB_2769335
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using EPHA3 Rabbit pAb (CAB8414) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
