The Eph receptor B3 (EPHB3) Monoclonal Antibody (CAB19229) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in various applications such as Western blot and immunohistochemistry.EphB3 is a transmembrane receptor tyrosine kinase that plays a crucial role in regulating cell adhesion, migration, and axon guidance. Dysregulation of EphB3 signaling has been implicated in various diseases, including cancer, neurological disorders, and cardiovascular conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Eph receptor B3 (EPHB3) Monoclonal Antibody
SKU:
CAB19229
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC2384
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
SELA WTSH PESG WEEV SGYD EAMN PIRT YQVC NVRE SSQN NWLR TGFI WRRD VQRV YVEL KFTV RDCN SIPN IPGS CKET FNLF YYEA DSDV ASAS SPFW M
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EK2, ETK2, HEK2, TYRO6, Eph receptor B3 (EPHB3)
Positive Sample:
HeLa, HT-29, SH-SY5Y, Mouse brain, Rat brain
Cellular Localization:
Cell Membrane, Cell Projection, Single-Pass Type I Membrane Protein, Dendrite.
Calculated MW:
110kDa
Observed MW:
110kDa
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. This gene encodes a receptor for ephrin-B family members.
Purification Method
Affinity purification
Gene ID
2049
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Eph receptor B3 (EPHB3) Rabbit mAb (CAB19229) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of lysates from Mouse brain, using Eph receptor B3 (EPHB3) Rabbit mAb (CAB19229) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
