The EPN1 Antibody (CAB17695) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit-derived antibody is highly specific to human samples and has been validated for use in Western blot applications. By binding to the EPN1 protein, this antibody enables detection and analysis in various cell types, making it ideal for studies in cell biology, neuroscience, and cancer research.EPN1 is essential for the formation of clathrin-coated vesicles, which are involved in the internalization of cell surface receptors and other molecules.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
EPN1 Antibody
SKU:
CAB17695
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
LVAL LRDE DRLR EERA HALK TKEK LAQT ATAS SAAV GSGP PPEA EQAW PQS
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EPN1
Positive Sample:
U-87MG, Mouse lung, Mouse liver, Mouse kidney, Rat testis, Rat brain
This gene encodes a member of the epsin protein family. The encoded protein binds clathrin and is involved in the endocytosis of clathrin-coated vesicles. Loss of function of this gene is associated with reduced tumor growth and progression in certain cancer types.
Purification Method
Affinity purification
Gene ID
29924
RRID
AB_2769341
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using EPN1 Rabbit pAb (CAB17695) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using EPN1 Rabbit pAb (CAB17695) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
