The ErbB4/HER4 Monoclonal Antibody (CAB19047) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. RRID AB_2862540 Gene ID 2066 Swiss Prot Synonym HER4; ALS19; p180erbB4; ErbB4/HER4
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
ErbB4/HER4 Monoclonal Antibody
SKU:
CAB19047
Size:
100μL, 20μL
Reactivity:
Mouse, Rat
Clone Number:
ARC2639
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HER4, ALS19, p180erbB4, ErbB4/HER4
Positive Sample:
Mouse brain, Rat brain
Cellular Localization:
Cell Membrane, Mitochondrion, Nucleus, Single-Pass Type I Membrane Protein.
Calculated MW:
147kDa
Observed MW:
180kDa
This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. RRID AB_2862540 Gene ID 2066 Swiss Prot Synonym HER4; ALS19; p180erbB4; ErbB4/HER4
Purification Method:
Affinity purification
Gene ID:
2066
RRID:
AB_2862540
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ErbB4/HER4 Rabbit mAb (CAB19047) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
