Description
ERGIC3 Antibody (PACO42654)
The ERGIC3 Antibody (PAC042654) is a valuable tool for researchers studying ERGIC3, an endoplasmic reticulum-Golgi intermediate compartment protein known to play a role in protein transport and sorting. This polyclonal antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for Western blot applications. By targeting the ERGIC3 protein, this antibody allows for precise detection and analysis of ERGIC3 expression in various cell types, making it an essential reagent for investigations in cell biology and protein trafficking.
ERGIC3 is a key player in the secretory pathway, facilitating the transport of cargo proteins from the endoplasmic reticulum to the Golgi apparatus. Its involvement in protein sorting and trafficking makes ERGIC3 a critical component in cellular processes related to protein maturation and secretion. Understanding the function of ERGIC3 is essential for unraveling the mechanisms underlying intracellular protein transport and organelle communication, with implications for research in cell biology and neurodegenerative diseases like Alzheimer's and Parkinson's.
| Antibody Name: | ERGIC3 Antibody (PACO42654) |
| Antibody SKU: | PACO42654 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Endoplasmic reticulum-Golgi intermediate compartment protein 3 protein (47-341AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot. All lanes: ERGIC3 antibody at 2µg/ml. Lane 1: HepG2 whole cell lysate. Lane 2: 293T whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: MCF-7 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 44, 27 kDa. Observed band size: 44 kDa. |
 | Immunofluorescent analysis of Hela cells using PACO42654 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Immunohistochemistry of paraffin-embedded human colon tissue using PACO42654 at dilution of 1:100. |
| Background: | Possible role in transport between endoplasmic reticulum and Golgi. |
| Synonyms: | Endoplasmic reticulum-Golgi intermediate compartment protein 3 (Serologically defined breast cancer antigen NY-BR-84), ERGIC3, C20orf47 ERV46 SDBCAG84 |
| UniProt Protein Function: | SDBCAG84: Possible role in transport between endoplasmic reticulum and Golgi. Belongs to the ERGIC family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Motility/polarity/chemotaxis; Vesicle; Membrane protein, integral Chromosomal Location of Human Ortholog: 20pter-q12 Cellular Component: endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; Golgi apparatus; integral to membrane; membrane Biological Process: vesicle-mediated transport |
| UniProt Code: | Q9Y282 |
| NCBI GenInfo Identifier: | 37999823 |
| NCBI Gene ID: | 51614 |
| NCBI Accession: | Q9Y282.1 |
| UniProt Secondary Accession: | Q9Y282,Q5JWS3, Q6ZWP7, Q9H276, Q9P1L3, |
| UniProt Related Accession: | Q9Y282 |
| Molecular Weight: | 43,772 Da |
| NCBI Full Name: | Endoplasmic reticulum-Golgi intermediate compartment protein 3 |
| NCBI Synonym Full Names: | ERGIC and golgi 3 |
| NCBI Official Symbol: | ERGIC3Â Â |
| NCBI Official Synonym Symbols: | Erv46; CGI-54; PRO0989; C20orf47; NY-BR-84; SDBCAG84; dJ477O4.2Â Â |
| NCBI Protein Information: | endoplasmic reticulum-Golgi intermediate compartment protein 3 |
| UniProt Protein Name: | Endoplasmic reticulum-Golgi intermediate compartment protein 3 |
| UniProt Synonym Protein Names: | Serologically defined breast cancer antigen NY-BR-84 |
| Protein Family: | Endoplasmic reticulum-Golgi intermediate compartment protein |
| UniProt Gene Name: | ERGIC3Â Â |
| UniProt Entry Name: | ERGI3_HUMAN |
