The ESAM Antibody (CAB12210) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and has been validated for use in Western blot applications, allowing for the detection and analysis of ESAM protein in human samples. Its high reactivity and specificity make it an ideal tool for studying ESAM expression and function in different cell types, particularly in the fields of vascular biology and immune response regulation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
ESAM Antibody
SKU:
CAB12210
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
W117m, ESAM
Positive Sample:
HT-29, HepG2, 293T, A-549
Cellular Localization:
Cell Junction, Cell Membrane, Single-Pass Type I Membrane Protein, Adherens Junction, Tight Junction.
Calculated MW:
41kDa
Observed MW:
60kDa
Enables cell-cell adhesion mediator activity. Involved in several processes, including bicellular tight junction assembly; cell-cell adhesion; and regulation of actin cytoskeleton organization. Located in cell-cell junction and plasma membrane. Part of protein-containing complex.
Purification Method
Affinity purification
Gene ID
90952
RRID
AB_2759091
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ESAM Rabbit pAb (CAB12210) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
