The ESD Antibody (CAB6228) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity when used with human samples in various applications, with particular validation for immunohistochemistry techniques. By binding to ESD, it enables precise detection and analysis of this protein in different cell types, making it an essential resource for investigations in the fields of cell biology and cancer research.ESD, or Epithelial Stromal Interaction 1, plays a crucial role in maintaining the integrity and function of epithelial tissues by regulating cell proliferation and differentiation.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ESD Antibody
SKU:
CAB6228
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FGH, ESD
Positive Sample:
MCF7, Jurkat, HepG2, 293T, Mouse kidney
Cellular Localization:
Cytoplasm, Cytoplasmic Vesicle.
Calculated MW:
31kDa
Observed MW:
36kDa
This gene encodes a serine hydrolase that belongs to the esterase D family. The encoded enzyme is active toward numerous substrates including O-acetylated sialic acids, and it may be involved in the recycling of sialic acids. This gene is used as a genetic marker for retinoblastoma and Wilson's disease.
Purification Method
Affinity purification
Gene ID
2098
RRID
AB_2766837
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ESD Rabbit pAb (CAB6228) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human trachea using ESD Rabbit pAb (CAB6228) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
