The ESE1/ELF3 Monoclonal Antibody (CAB5236) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated from rabbit cells, exhibits high specificity and sensitivity when detecting ESE1 in human samples, making it perfect for Western blot applications.ESE1, also known as epithelium-specific ETS transcription factor-1, is involved in various biological processes, including cell differentiation, apoptosis, and cancer development.
This antibody is validated for use in WB, ChIP, ELISA, CUT&Tag applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ESE1/ELF3 Monoclonal Antibody
SKU:
CAB5236
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1191
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
ChIP
5μg antibody for 10μg-15μg of Chromatin
CUT&Tag
10⁵ cells /1 μg
Synonyms:
ERT, ESX, EPR-1, ESE-1, ESE1/ELF3
Positive Sample:
A549, A-431, Mouse lung, Rat kidney
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
41kDa
Observed MW:
42kDa
Enables DNA-binding transcription activator activity, RNA polymerase II-specific and sequence-specific double-stranded DNA binding activity. Involved in inflammatory response; negative regulation of transcription, DNA-templated; and positive regulation of transcription by RNA polymerase II. Located in Golgi apparatus; cytosol; and nucleoplasm.
Purification Method
Affinity purification
Gene ID
1999
RRID
AB_2863496
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ESE1/ELF3 Rabbit mAb (CAB5236) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Chromatin immunoprecipitation analysis of extracts of Hep G2 cells, using ESE1/ELF3 antibody (CAB5236) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
