The ESE1/ELF3 Monoclonal Antibody (CAB5236) is a high-quality antibody developed for reliable detection and analysis of target proteins. Enables DNA-binding transcription activator activity, RNA polymerase II-specific and sequence-specific double-stranded DNA binding activity. Involved in inflammatory response; negative regulation of transcription, DNA-templated; and positive regulation of transcription by RNA polymerase II. Located in Golgi apparatus; cytosol; and nucleoplasm. RRID AB_2863496 Gene ID 1999 Swiss Prot Synonym ERT; ESX; EPR-1; ESE-1; ESE1/ELF3
This antibody is validated for use in WB, ChIP, ELISA, CUT&Tag applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ESE1/ELF3 Monoclonal Antibody
SKU:
CAB5236
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1191
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBChIPELISACUT&Tag
Recommended Dilution:
WB
1:1000 - 1:6000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. Ch
IP
5μg antibody for 10μg-15μg of Chromatin
CUT
&Tag 10⁵ cells /1 μg
Synonyms:
ERT, ESX, EPR-1, ESE-1, ESE1/ELF3
Positive Sample:
A549, A-431, Mouse lung, Rat kidney
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
41kDa
Observed MW:
42kDa
Enables DNA-binding transcription activator activity, RNA polymerase II-specific and sequence-specific double-stranded DNA binding activity. Involved in inflammatory response; negative regulation of transcription, DNA-templated; and positive regulation of transcription by RNA polymerase II. Located in Golgi apparatus; cytosol; and nucleoplasm. RRID AB_2863496 Gene ID 1999 Swiss Prot Synonym ERT; ESX; EPR-1; ESE-1; ESE1/ELF3
Purification Method:
Affinity purification
Gene ID:
1999
RRID:
AB_2863496
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ESE1/ELF3 Rabbit mAb (CAB5236) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Chromatin immunoprecipitation analysis of extracts of Hep G2 cells, using ESE1/ELF3 antibody (CAB5236) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
