The ETFDH Antibody (CAB6585) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the ETFDH protein, this antibody allows for accurate detection and analysis in a variety of cell types.ETFDH is essential for the breakdown of fatty acids and plays a crucial role in energy production within the cell. Mutations in the ETFDH gene have been linked to disorders such as riboflavin-responsive multiple acyl-CoA dehydrogenase deficiency (RR-MADD), a rare metabolic disorder that affects the body's ability to break down fats and proteins.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ETFDH Antibody
SKU:
CAB6585
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MADD, ETFQO, ETFDH
Positive Sample:
Mouse liver, Mouse heart, Rat liver, Rat heart
Cellular Localization:
Mitochondrion Inner Membrane.
Calculated MW:
68kDa
Observed MW:
64kDa
This gene encodes a component of the electron-transfer system in mitochondria and is essential for electron transfer from a number of mitochondrial flavin-containing dehydrogenases to the main respiratory chain. Mutations in this gene are associated with glutaric acidemia. Alternatively spliced transcript variants that encode distinct isoforms have been observed.
Purification Method
Affinity purification
Gene ID
2110
RRID
AB_2767178
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ETFDH Rabbit pAb (CAB6585) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
