The ETS1 Monoclonal Antibody (CAB19603) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. RRID AB_2862692 Gene ID 2113 Swiss Prot Synonym p54; ETS-1; EWSR2; c-ets-1; ETS1
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
ETS1 Monoclonal Antibody
SKU:
CAB19603
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC0082
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:4000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p54, ETS-1, EWSR2, c-ets-1, ETS1
Positive Sample:
A549, Jurkat, MCF7
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
50kDa
Observed MW:
50kDa
This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. RRID AB_2862692 Gene ID 2113 Swiss Prot Synonym p54; ETS-1; EWSR2; c-ets-1; ETS1
Purification Method:
Affinity purification
Gene ID:
2113
RRID:
AB_2862692
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ETS1 Rabbit mAb (CAB19603) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
