The EV71 3D Monoclonal Antibody (CAB22651) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody specifically targets the 3D protein of EV71, which plays a critical role in viral replication and infectivity. Produced using hybridoma technology, the EV71 3D Monoclonal Antibody is highly specific and sensitive, allowing for accurate detection and quantification of EV71 in various samples. It is validated for use in immunofluorescence, ELISA, and other detection methods, making it versatile for different experimental needs.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
EV71 3D Monoclonal Antibody
SKU:
CAB22651
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC58400
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:20000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Sample:
HeLa transfected with EV71 3D
Calculated MW:
51kDa
Observed MW:
60kDa
Purification Method
Affinity purification
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and HeLa cells transfected with EV71 3D using EV71 3D Rabbit mAb (CAB22651) at 1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
