The EXT1 Polyclonal Antibody (CAB24353) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the EXT1 protein, this antibody enables accurate detection and analysis in various cell types, making it an essential component for studies in glycobiology and extracellular matrix research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
EXT1 Polyclonal Antibody
SKU:
CAB24353
Size:
20μL, 100μL
Reactivity:
Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
EXT, LGS, TTV, LGCR, TRPS2, EXT1
Positive Sample:
Rat spleen
Cellular Localization:
Endoplasmic Reticulum Membrane, Golgi Apparatus Membrane, Single-Pass Type Ii Membrane Protein.
Calculated MW:
86kDa
Observed MW:
86kDa
This gene encodes an endoplasmic reticulum-resident type II transmembrane glycosyltransferase involved in the chain elongation step of heparan sulfate biosynthesis. Mutations in this gene cause the type I form of multiple exostoses.
Purification Method
Affinity purification
Gene ID
2131
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using EXT1 Rabbit pAb (CAB24353) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
