The F12 Polyclonal Antibody (CAB1691) is a valuable tool for research involving the F12 protein, a component of the coagulation cascade that plays a vital role in blood clotting. This antibody, produced in rabbits, is highly specific to human samples and has been verified for use in Western blot applications. It targets the F12 protein, allowing for accurate detection and analysis in a variety of cell types, making it well-suited for investigations in hematology and thrombosis research.F12, also known as coagulation factor XII, is essential for the initiation of the intrinsic pathway of blood coagulation. Dysregulation of F12 activity can lead to abnormal clot formation, contributing to conditions such as thrombosis and clotting disorders.
Research into the function and regulation of F12 is crucial for developing interventions to manage these disorders and improve patient outcomes.By utilizing the F12 Polyclonal Antibody (CAB1691), researchers can further explore the role of F12 in clot formation and other physiological processes, ultimately advancing our understanding of hemostasis and thrombotic diseases. Its specificity and reliability make it a valuable asset for studies aiming to uncover new insights into blood coagulation mechanisms and potential therapeutic targets.
Product Name:
F12 Rabbit Polyclonal Antibody
SKU:
CAB1691
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 336-615 of human F12 (NP_000496.2).
This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged.
Purification Method:
Affinity purification
Gene ID:
2161
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using F12 Rabbit pAb (CAB1691) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
