The F2R Antibody (CAB5641) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been extensively validated for use in Western blot applications. By specifically binding to the F2R protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in hemostasis, thrombosis, and inflammatory processes. F2R, also known as the protease-activated receptor 1 (PAR-1), plays a crucial role in mediating cellular responses to thrombin, a key player in blood clotting and inflammation.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
F2R Antibody
SKU:
CAB5641
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TR, HTR, CF2R, PAR1, PAR-1, F2R
Positive Sample:
Mouse heart, Rat lung
Cellular Localization:
Cell Membrane, Multi-Pass Membrane Protein.
Calculated MW:
47kDa
Observed MW:
70kDa
Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
2149
RRID
AB_2766401
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using F2R Rabbit pAb (CAB5641) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
