The FAN1 Polyclonal Antibody (PACO58997) is a vital tool for researchers studying the FAN1 protein, which plays a key role in DNA repair and maintenance. This antibody, produced in rabbits, exhibits a high level of reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the FAN1 protein, researchers can accurately detect and analyze this important molecule in various cell types.FAN1 is known for its involvement in DNA inter-strand crosslink repair and is essential for genome stability. Dysregulation of FAN1 has been linked to various diseases, including cancer and other genomic instability disorders.
By studying the function of FAN1, researchers can gain valuable insights into these conditions and potentially develop new therapeutic approaches for treating them.Overall, the FAN1 Polyclonal Antibody (PACO58997) is a reliable and indispensable tool for researchers interested in unraveling the complexities of DNA repair mechanisms and the implications for human health. Its high specificity and sensitivity make it an ideal choice for studies in molecular biology, genetics, and oncology.
Western Blot. Positive WB detected in: PC-3 whole cell lysate. All lanes: FAN1 antibody at 5.3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 115, 60 kDa. Observed band size: 115 kDa.
IHC image of PACO58997 diluted at 1:300 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions. Probably keeps excising with 3'-flap annealing until it reaches and unhooks the ICL. Acts at sites that have a 5'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3' flap. Also has endonuclease activity toward 5'-flaps.
FAN1: Nuclease required for maintenance of chromosomal stability. Plays a key role in DNA repair of DNA interstrand cross-links (ICL) by being recruited to sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL- induced DNA breaks by being required for efficient homologous recombination, possibly in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Has both endonuclease activity toward 5'-flaps and 5'-exonuclease activity: may act in concert with the 3'-flap- specific enzymes to unhook the ICL by cleaving the lagging-strand template. Belongs to the FAN1 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 3.1.4.1; Deoxyribonuclease; Ubiquitin conjugating system; DNA repair, damageChromosomal Location of Human Ortholog: 15q13.2-q13.3Cellular Component: cytoplasm; intercellular bridge; nucleoplasm; nucleusMolecular Function: 5'-3' exonuclease activity; 5'-flap endonuclease activity; magnesium ion binding; protein binding; ubiquitin bindingBiological Process: DNA repair; double-strand break repair via homologous recombination; nucleotide-excision repair; nucleotide-excision repair, DNA incisionDisease: Interstitial Nephritis, Karyomegalic
UniProt Protein Details:
NCBI Summary:
This gene plays a role in DNA interstrand cross-link repair and encodes a protein with 5' flap endonuclease and 5'-3' exonuclease activity. Mutations in this gene cause karyomegalic interstitial nephritis. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Feb 2016]
