Description
FANCD2 Antibody (CAB19311)
The FANCD2 Antibody (CAB19311) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the FANCD2 protein, the antibody enables the detection and analysis of this key player in DNA damage response pathways in a variety of cell types.FANCD2 is a key component of the Fanconi Anemia pathway, a complex network of proteins responsible for repairing DNA damage and preventing the accumulation of mutations.
This antibody is validated for use in WB, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
| Product Name: | FANCD2 Antibody |
| SKU: | CAB19311 |
| Size: | 20μL, 100μL |
| Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||||||
| Sequence: | MVSK RRLS KSED KESL TEDA SKTR KQPL SKKT KKSH IANE VEEN DSIF VKLL KISG IILK TGES QNQL AVDQ IAFQ KKLF QTLR RHPS YPKI IEEF VSGL ESYI EDED SFRN CLLS CERL QDEE ASMG ASYS KSLI KLLL GIDI LQPA IIKT LFEK LPEY FFEN KNSD EINI PRLI VSQL KWLD RVVD GKDL TTKI MQLI SIAP ENLQ HDII TSLP EILG DSQH ADVG KELS DLLI ENTS LTVP ILDV LSSL RLDP NFLL KVRQ LVMD KLSS IRLE DLPV IIKF ILHS VTA | ||||||||
| Tested Applications: | WB IF/ICC IP ELISA | ||||||||
| Recommended Dilution: |
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| Synonyms: | FA4, FAD, FACD, FAD2, FA-D2, FANCD, FANCD2 |
| Positive Sample: | K-562, HeLa, A-549, C6, Mouse testis |
| Cellular Localization: | Nucleus. |
| Calculated MW: | 164kDa |
| Observed MW: | 164kDa |
The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repair. Alternative splicing results in multiple transcript variants.
| Purification Method | Affinity purification |
| Gene ID | 2177 |
| RRID | AB_2862639 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using FANCD2 Rabbit pAb (CAB19311) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s. |
 | Immunofluorescence analysis of C6 cells using FANCD2 Rabbit pAb (CAB19311) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
