The Fibroblast activation protein-alpha (FAP) Antibody (CAB6349) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. RRID AB_2766951 Gene ID 2191 Swiss Prot Synonym FAPA; SIMP; DPPIV; FAPalpha; Fibroblast activation protein-α (FAP)
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Cell Membrane, Cell Projection, Cell Surface, Cytoplasm, Membrane, Secreted, Single-Pass Type Ii Membrane Protein, Single-Pass Type Ii Membrane Protein, Invadopodium Membrane, Lamellipodium Membrane, Ruffle Membrane.
Calculated MW:
88kDa
Observed MW:
90kDa/95kDa
The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. RRID AB_2766951 Gene ID 2191 Swiss Prot Synonym FAPA; SIMP; DPPIV; FAPalpha; Fibroblast activation protein-α (FAP)
Purification Method:
Affinity purification
Gene ID:
2191
RRID:
AB_2766951
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide,pH7.3.
Western blot analysis of various lysates using Fibroblast activation protein-α (FAP) Rabbit pAb (CAB6349)at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Negative control (NC): SK-MEL-28 Exposure time: 30s.
Immunofluorescence analysis of PC-12 cells using Fibroblast activation protein-α (FAP) Rabbit pAb (CAB6349) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
