The Fas Antibody (CAB0233) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it suitable for Western blot applications. By binding to the Fas protein, this antibody allows for the detection and analysis of Fas expression in various cell types, making it ideal for investigations in immunology, cancer research, and other fields where apoptosis and immune regulation are key factors.Fas, also known as CD95, is a crucial mediator of programmed cell death and plays a significant role in immune system function and development. Dysregulation of the Fas signaling pathway has been linked to various diseases, including cancer, autoimmune disorders, and neurodegenerative conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Fas Antibody
SKU:
CAB0233
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Cell Membrane, Secreted, Single-Pass Type I Membrane Protein.
Calculated MW:
9-38kDa
Observed MW:
38-55kDa
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.
Purification Method
Affinity purification
Gene ID
355
RRID
AB_2757046
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Fas Rabbit pAb (CAB0233) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
