The FBXL12 Antibody (CAB14589) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the FBXL12 protein, this antibody enables accurate detection and analysis in a variety of cell types.FBXL12 is known for its role in the ubiquitin-proteasome pathway, where it targets specific proteins for degradation, thus influencing various cellular functions such as cell cycle progression and gene expression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
FBXL12 Antibody
SKU:
CAB14589
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Fbl12, FBXL12
Positive Sample:
NIH/3T3, SW480, 293T, A-431, HeLa
Cellular Localization:
Cytosol, Nucleus.
Calculated MW:
37kDa
Observed MW:
37kDa
Members of the F-box protein family, such as FBXL12, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).
Purification Method
Affinity purification
Gene ID
54850
RRID
AB_2761463
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using FBXL12 Rabbit pAb (CAB14589) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
