The FBXO31 Antibody (CAB12888) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the FBXO31 protein, this antibody enables detection and analysis in various cell types, making it an essential reagent for studies in cell biology and cancer research.FBXO31, a member of the F-box protein family, plays a critical role in regulating the turnover of specific proteins within the cell, thereby influencing various cellular pathways and processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
FBXO31 Antibody
SKU:
CAB12888
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FBX14, Fbx31, MRT45, FBXO14, pp2386, FBXO31
Positive Sample:
Mouse brain, Rat brain
Cellular Localization:
Centrosome, Cytosol.
Calculated MW:
61kDa
Observed MW:
60kDa
This gene is a member of the F-box family. Members are classified into three classes according to the substrate interaction domain, FBW for WD40 repeats, FBL for leucing-rich repeats, and FBXO for other domains. This protein, classified into the last category because of the lack of a recognizable substrate binding domain, has been proposed to be a component of the SCF ubiquitination complex. It is thought to bind and recruit substrate for ubiquitination and degradation. This protein may have a role in regulating the cell cycle as well as dendrite growth and neuronal migration. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
79791
RRID
AB_2759731
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using FBXO31 Rabbit pAb (CAB12888) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
