The FBXO7 Antibody (CAB7464) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to FBXO7, this antibody allows for the detection and analysis of the protein in various cell types, making it particularly useful for investigations in molecular biology and neurodegenerative diseases.FBXO7 is a key player in the ubiquitin-proteasome pathway, which is responsible for targeting proteins for degradation and maintaining cellular homeostasis.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
FBXO7 Antibody
SKU:
CAB7464
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
FBX, FBX7, PKPS, FBX07, PARK15, FBXO7
Positive Sample:
U2OS, 293T
Cellular Localization:
Cytoplasm, Mitochondrion, Nucleus, Cytosol.
Calculated MW:
59kDa
Observed MW:
65-72kDa
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it may play a role in regulation of hematopoiesis. Alternatively spliced transcript variants of this gene have been identified with the full-length natures of only some variants being determined.
Purification Method
Affinity purification
Gene ID
25793
RRID
AB_2767995
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using FBXO7 Rabbit pAb (CAB7464) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 120s.
Immunofluorescence analysis of A549 cells using FBXO7 Rabbit pAb (CAB7464).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution.
